Tet On Promoter
TETRACYCLINE (TET) INDUCIBLE EXPRESSION - ADDGENE
16 rows Tetracycline-dependent promoters are developed by placing a … ...
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TET-ON SYSTEMS FOR DOXYCYCLINE-INDUCIBLE GENE EXPRESSION
The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes in eukaryotic cells in diverse settings, varying from basic … ...
Author Atze T. Das, Liliane Tenenbaum, Ben BerkhoutAuthors Atze T. Das, Liliane Tenenbaum, Ben BerkhoutPublish Year 2016DOI 10.2174/1566523216666160524144041
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Expand All Mechanism of Tet-inducible expression Why use Tet-On 3G systems? The pTRE3G inducible promoter: tetracycline-inducible expression, lowest basal activity level The Tet-On 3G transcriptional … ...
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Get CodeOPTIMISATION OF TET-ON INDUCIBLE SYSTEMS FOR SLEEPING …
Aug 4, 2020 5 Altmetric Metrics Abstract Regulated expression of genetic elements that either encode polypeptides or various types of functional RNA is a fundamental goal … ...
Author S. M. Ali Hosseini Rad, Aarati Poudel, Grace Min Yi Tan, Alexander D. McLellanPublish Year 2020
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Tet-On Systems For Doxycycline-inducible Gene Expression The tetracycline-controlled Tet-Off and Tet-On gene expression systems are used to regulate the activity of genes … ...
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TET-ONE TECHNOLOGY OVERVIEW - TAKARA BIO
PTRE3GS inducible promoter The inducible promoter PTRE3GS provides for very low basal expression and high maximal expression after induction (Loew et al. 2010). It consists of seven repeats of a 19-bp tet operator … ...
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Get CodeINTRODUCTION TO TET EXPRESSION SYSTEMS - THE JACKSON …
1. Baron U, Bujard H. 2000. Tet repressor-based system for regulated gene expression in eukaryotic cells: Principles and advances. Methods Enzymol 327: 401-21. 2. Furth P et … ...
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Get CodeAN ALL-IN-ONE, TET-ON 3G INDUCIBLE PIGGYBAC SYSTEM FOR …
May 8, 2017 The second promoter, a constitutive EF1α promoter, controls expression of a resistance gene for drug blasticidin (Bsd) and the Tet-On 3G transactivator protein … ...
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Get CodeTET PROMOTER (GNOXN5) | DOULIX
The tet promoter is found at the beginning of the tetracycline resistance region in plasmids containing class C resistance determinants, like pACYC184, pBR322 or pSC101. It … ...
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TET-ON 3G TETRACYCLINE-INDUCIBLE EXPRESSION SYSTEMS
Overview Lowest background and highest sensitivity Highest fold induction 14 kit combinations— click here for available options Monitor induced expression using fluorescent proteins and a bidirectional promoter ...
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Get CodeTET-ON AND TET-OFF SYSTEMS: SECOND GENERATION - TAKARA BIO
Tet-On and Tet-Off systems: second generation Tet-On and Tet-Off brands are the highest-performing and most-cited inducible mammalian expression systems. Since the orignal … ...
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Get CodeA TUNABLE DUAL-INPUT SYSTEM FOR ON-DEMAND DYNAMIC GENE
Oct 2, 2019 A major limitation of inducible promoters is the significant time delay in switching proteins OFF and ON when using Tet-On and Tet-Off systems, respectively … ...
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Get CodeTET-ON® 3G INDUCIBLE EXPRESSION SYSTEMS USER MANUAL - TAKARA BIO
The Tet-On 3G Systems are inducible gene expression systems for mammalian cells. Target cells that express the Tet-On 3G transactivator protein and contain a gene of … ...
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TET-ONE™ INDUCIBLE EXPRESSION SYSTEM USER MANUAL - TAKARA BIO
PTRE3GS Inducible Promoter The inducible promoter PTRE3G provides for very low basal expression and high maximal expression after induction (Loew et. al., 2010). It consists … ...
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Get CodeIMPROVED TET-RESPONSIVE PROMOTERS WITH MINIMIZED BACKGROUND …
Nov 24, 2010 The novel composite Ptet promoters introduced here will further expand the utility of the Tet system. Background The Tet system is the most widely used inducible … ...
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Get CodeTET: PRINCIPLES & COMPONENTS
Ptet is a synthetic promoter responsive to both tTA and rtTA. It is comprised of a minimal RNA polymerase II promoter (transcriptionally silent in the absence of additional … ...
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Nov 24, 2010 PMCID: PMC3002914 DOI: 10.1186/1472-6750-10-81 Abstract Background: The performance of the tetracycline controlled transcriptional activation system (Tet … ...
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OPTIMIZATION OF THE TET-ON SYSTEM FOR REGULATED GENE ... - NATURE
May 25, 2006 Fusion of the VP16 activation domain of herpes simplex virus to TetR resulted in the transcriptional activator tTA, which induces gene expression from tetO … ...
Category: Domain
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Get CodeINDUCIBLE GENE EXPRESSION VECTOR (TET-ON) | VECTORBUILDER
Jul 12, 2023 The Tet-On inducible system is a powerful tool to control the timing of expression of the gene (s) of interest (GOI) in mammalian cells. ...
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Get CodeEFFICIENT TISSUE-TYPE SPECIFIC EXPRESSION OF TARGET GENES IN A
Jan 14, 2020 The ability of the tTA to activate tet-operator/promoter (TetO)-driven effector transgenes is controlled by administration of doxycycline (Dox), which is an effective and … ...
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Nov 24, 2010 Additional file 1 Tet operator heptamer of the Ptet-T series of Ptet promoters. The identical tet operator sequences are underlined, their imperfect inverted … ...
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OPTIMIZATION OF THE TET-ON SYSTEM FOR INDUCIBLE EXPRESSION OF RAGE
The CMV promoter of pIRES-RAGE-EGFP was replaced with TREtight, a modified Tet-responsive promoter that contains a Tet-response element with seven direct repeats of … ...
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