Tetracycline Inducible Expression System Deal
TETRACYCLINE-INDUCIBLE EXPRESSION SYSTEMS - TAKARA BIO
Tetracycline-controlled transcriptional activation is a method of inducible gene expression where transcription is reversibly turned on or off in the presence of the antibiotic tetracycline or one of its derivatives (e.g. doxycycline). Tetracycline-controlled gene expression is based upon the mechanism of resistance to tetracycline antibiotic treatment found in gram-negative bacteria. … ...
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TETRACYCLINE-CONTROLLED TRANSCRIPTIONAL ACTIVATION - WIKIPEDIA
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Estimated Reading Time 8 mins
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May 23, 2023 Here, we implemented the well-characterized tetracycline-inducible expression system in two industrially important lactic acid bacteria, Lactococcus lactis … ...
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Get CodeTETRACYCLINE INDUCIBLE EXPRESSION—TET-ONE SYSTEMS - TAKARA BIO
Until now, the best inducible mammalian expression systems have all required a two-vector format. Tet-One inducible expression systems are the only commercially available single-vector systems that can deliver a >10,000-fold difference in expression levels between induced and uninduced states. ...
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Get CodeNEW REGULATORS OF THE TETRACYCLINE‐INDUCIBLE GENE …
Sep 11, 2022 The tetracycline repressor (tetR)‐regulated system is a widely used tool to specifically control gene expression in mammalian cells. ...
DOI 10.1002/2211-5463.13482Published 2022/10Publication FEBS Open Bio. 2022 Oct; 12(10)
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AN IMPROVED TET-ON SYSTEM IN MICRORNA OVEREXPRESSION …
Jun 10, 2019 Tetracycline inducible system (Tet-on and Tet-off) has been extensively applied in biological study in vitro and in vivo due to it having a number of advantages, … ...
Author Kang Kang, Lian Huang, Qing Li, Xiaoyun Liao, Quanjin Dang, Yi Yang, Jun Luo, Yan Zeng, Li Li, Demin...Estimated Reading Time 7 minsPublish Year 2019
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The tetracycline repressor (tetR)-regulated system is a widely used tool to specifically control gene expression in mammalian cells. Based on this system, we generated a … ...
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Get CodeA TUNABLE DUAL-INPUT SYSTEM FOR ON-DEMAND DYNAMIC …
Oct 2, 2019 The introduction of inducible promoters has allowed gene expression control using orthogonal molecules, facilitating its rapid and reversible manipulation to study … ...
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Get CodeA TETRACYCLINE-DEPENDENT RIBOZYME SWITCH ALLOWS …
Jan 30, 2019 Ribozyme insertion into the 3’-UTR converts any gene of interest into a tetracycline-inducible gene allowing temporal and, by using tissue-selective promoters, … ...
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TETRACYCLINE-INDUCIBLE EXPRESSION SYSTEMS FOR THE …
Oct 30, 2004 The most often used tetracycline-regulated transgenic mice system requires the generation of two transgenic strains, one carrying an inducible promoter and the … ...
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Oct 30, 2004 The tetracycline-inducible (Tet-based) systems have been shown to be an effective tool for examining the contribution of specific gene functions to the induction and … ...
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Oct 30, 2004 The results showed that a tetracycline-inducible expression system in transgenic animals might be a promising solution to minimize physiological disturbances … ...
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Get CodeINDUCIBLE PROTEIN EXPRESSION—T-REX SYSTEM - THERMO FISHER …
Because tetracycline-regulated expression in the T-REx System is based on a repression/derepression mechanism, the amount of Tet repressor that is expressed … ...
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TET B OR NOT TET B: ADVANCES IN TETRACYCLINE-INDUCIBLE GENE …
Feb 2, 1999 Be it the B class, or another class of tetracycline (tet) repressor, the utility and specificity of transcriptional regulators based on this family of prokaryotic DNA binding … ...
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Get CodeIMPROVEMENT OF THE REVERSE TETRACYCLINE TRANSACTIVATOR BY ... - NATURE
Jun 21, 2016 We also created a β-estradiol inducible expression system 26 to gain direct control of rtTA-M2 expression and used the promoter with an extra TetR binding site, P … ...
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Get CodeTETRACYCLINE-INDUCIBLE EXPRESSION SYSTEMS WITH REDUCED BASAL …
Jan 1, 1999 Regulatable gene expression methods for mammalian cells allowing tight and specific regulation are rare. Using the regulatory elements of the Tn10-encoded tetracycline (tc) resistance operon from Escherichia coli (for review see 1), an inducible system of over five orders of magnitude was developed for HeLa cells.TetR is converted into a … ...
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Get CodeOPTIMISATION OF TET-ON INDUCIBLE SYSTEMS FOR SLEEPING BEAUTY …
Aug 4, 2020 Modified tetracycline-inducible systems represent the most widely used inducible system in eukaryotic systems, from yeast to human cells 5. The potential … ...
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STRUCTURE AND FUNCTION IN RHODOPSIN: A TETRACYCLINE-INDUCIBLE …
Oct 7, 2002 Inducible expression was found to require both tetracycline and sodium butyrate; the two components acting synergistically to give high-level expression of the … ...
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In recent years, some strategies derived from tetracycline-inducible system alone, as well as the combined use of Tet-based systems and Cre/lox P switching gene expression … ...
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We have utilized pharmacologically regulated gene expression systems to achieve fine control of gene expression levels which facilitate research in basic biology and … ...
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Feb 21, 2017 A tetracycline-inducible integrative expression system for Streptococcus pneumoniae Marina Meiers, Anke Laux, Daniela Eichinger, Anne Sexauer, Patrick Marx, … ...
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A MODIFIED TETRACYCLINE-REGULATED SYSTEM PROVIDES AUTOREGULATORY ... - PNAS
Jul 3, 1995 A system for tetracycline-regulated inducible gene expression was described recently which relies on constitutive expression of a tetracycline-cont... A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. | PNAS ...
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